Definition: A restriction enzyme is a DNA-cutting protein, found mainly in bacteria and archaea, that recognizes a specific short DNA sequence and cleaves the double helix at or near that site. By ...
We next determined which gene(s) on pTEF2 were responsible for the restriction of phi47. Of the 62 open reading frames (ORFs) encoded by pTEF2, approximately half are predicted to be involved in the ...
Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...
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